Generator
Part:BBa_K1486041:Design
Designed by: Ione Pla Puigsubirà Group: iGEM14_EPF_Lausanne (2014-09-27)
sfGFPC + CpxR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 1394 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1805 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The stop codon of the CpxR was deleted and a flexible linker (GGGGS) was added between the two proteins.
Source
The sources for this sequences are the CpxR coding sequence (BBa_K1486000) and the C-terminal split-sfGFP (BBa_K1486039)